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American Society of Microbiology ABMM Sample Questions:

1. A patient presents with perianal itching, particularly at night. Microscopic examination of cellophane tape preparation from the perianal area reveals oval-shaped eggs with one flattened side. The MOST likely causative organism is:

A) Trichuris trichiura
B) Ascaris lumbricoides
C) Enterobius vermicularis
D) Strongyloides stercoralis

2. The primary mechanism of action of aminoglycoside antibiotics, such as gentamicin, involves:

A) Disruption of bacterial cell membrane integrity
B) Inhibition of bacterial DNA gyrase and topoisomerase IV
C) Inhibition of bacterial protein synthesis by binding to the 30S ribosomal subunit
D) Inhibition of bacterial cell wall synthesis by binding to penicillin-binding proteins

3. A patient develops a febrile illness with jaundice and dark urine after receiving a blood transfusion.
Laboratory testing reveals an elevated level of unconjugated bilirubin and a positive direct Coombs test.
Serological testing is positive for antibodies against a protozoan parasite that infects erythrocytes. The MOST likely causative agent is:

A) Plasmodium falciparum
B) Toxoplasma gondii
C) Trypanosoma cruzi
D) Babesia microti

4. Microscopic examination of a trichrome-stained stool smear reveals amoebic trophozoites approximately 25
µm in diameter, containing ingested red blood cells. The nucleus, when visible, has fine, evenly distributed peripheral chromatin and a small, central karyosome. These features are diagnostic for:

A) Iodamoeba bütschlii
B) Entamoeba coli
C) Endolimax nana
D) Entamoeba histolytica

5. A clinical laboratory receives a cerebrospinal fluid (CSF) sample from a 65-year-old patient presenting with fever, headache, and nuchal rigidity. Gram stain reveals Gram-positive cocci in pairs and short chains. Culture on blood agar grows alpha-hemolytic colonies that are optochin-susceptible and bile soluble. Which organism is most likely identified?

A) Staphylococcus aureus
B) Enterococcus faecalis
C) Streptococcus agalactiae
D) Streptococcus pneumoniae

Solutions: